Fig 3.

Motif I sequence conservation among RquA orthologs compared with UbiE and UbiG orthologs in the same set of species. Amino acid conservation is depicted by using WebLogo (6). Boxes indicate the locations of signature motif I in each alignment, and asterisks indicate the positions in RquA orthologs that differ from the consensus signature motif I (30). Orthologs of RquA were identified as members of NCBI protein cluster CLSK588563 and were located in the following species: Aromatoleum aromaticum EbN1, Azoarcus sp. strain BH72, “Candidatus Accumulibacter phosphatis” clade IIA strain UW-1, Chromobacterium violaceum ATCC 12472, Laribacter hongkongensis HLHK9, Leptothrix cholodnii SP-6, Magnetococcus sp. strain MC-1, Methylocella silvestris BL2, Rhodoferax ferrireducens T118, and Rhodospirillum rubrum ATCC 11170. Orthologs of the E. coli UbiE and UbiG genes were identified in this set of species as the highest-scoring hits using BLASTp. The sequences were aligned by using ClustalW (42) to identify motif I.