Table 1.
Strains and plasmids used in this study
| E. coli strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| KM-D | Clinical E. coli isolate from an intestinal fistula, marR | 24 |
| PC1012 | KM-D ΔsoxS Δrob ΔmarA | 10 |
| DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Invitrogen |
| S17 λpir | lamB F−supE44 thi-1 thr-1 leuB6 lacY1 tonA21 hsdR hsdM recA pro (RP4 2-Tc::Mu::Km::Tn7) λpir | 49 |
| KM-DznuC | KM-D znuC | This study |
| PC1012znuC | PC1012 znuC::Cm | This study |
| PC1037 | PC1012::soxS (soxS restored to chromosome) | 10 |
| PC1038 | PC1012::rob | 10 |
| PC1033 | PC1012::marA | 10 |
| PC1005 | KM-D ΔsoxS | 10 |
| SIP468 | MC4100 aroB znuA::MudX | 37 |
| SIP775 | MC4100 aroB znuC::MudX | 37 |
| JW4023-5 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ−rph-1 Δ(rhaD-rhaB)568 ΔsoxS756::kan hsdR514 | 3 |
| JTG1078 | soxR105 zjc-2204::Tn10kan; overexpression of soxS | 14 |
| SIP468soxS | SIP468 transduced with ΔsoxS756::kan from JW4023-5 | This study |
| SIP775soxS | SIP775 transduced with ΔsoxS756::kan from JW4023-5 | This study |
| SIP468soxR105 | SIP468 transduced with soxR105 zjc-2204::kan from JTG1078 | This study |
| SIP775soxR105 | SIP775 transduced with soxR105 zjc-2204::kan from JTG1078 | This study |
| Plasmids | ||
| pGEM | High-copy-no. cloning vector (Apr) | Promega |
| pSR47s | R6KoriV RP4oriT sacB (Kmr) | 22 |
| pKD3 | Source of cat cassette (Cmr) | 13 |
| pNTR-SD | Ptac lacIq; low copy no. (Apr) | 45 |
| pZnuC | Intact znuC gene with promoter cloned into A/T site in pGEM (Apr) | This study |
| pZnuC::Cm | znuC disrupted with the cat cassette; pGEM(Apr Chlr) | This study |
| pSRznuC::Cm | NotI fragment from pZnuC::Cm cloned into NotI site of pSR47s (Cmr Kmr) | This study |
| pZnuACB | Intact znuACB locus with promoter cloned into A/T site in pGEM (Apr) | This study |
| pNTznuACB | NotI fragment of pZnuACB cloned into NotI site of pNTR-SD (Apr) | This study |
| pSXS-Cm | IPTG-inducible soxS gene | 1 |
a
IPTG, isopropyl-β-d-thiogalactopyranoside.