Table 2.
Differences in gene expression between strains KM-D and PC1012
| Gene | Test and RNA sourcea |
||
|---|---|---|---|
| Microarray analysis with murine kidneys | qRT-PCR |
||
| Murine kidneys | In vitro | ||
| znuC | 18.9 ± 8.1 | 9.9 ± 0.8 | 2.5 ± 0.5 |
| znuA | 0.42 ± 0.5 | 1.2 ± 0.2 | 1.4 ± 0.3 |
| zur | 1.03 ± 3.0 | 1.1 ± 0.1 | 1.2 ± 0.3 |
a
All mouse-derived cDNA was synthesized from RNA extracted from the infected kidneys of mice at day 2 postinfection. All in vitro results are from tests performed on cDNA synthesized from RNA isolated from bacteria grown to mid-log phase in M9 minimal medium.