Table 3.
Effects of removal or overexpression of soxS on znu expression in strain MC4100 as determined by β-galactosidase activity
| Strain | Ratio of LacZ activity ± SEMb |
|---|---|
| SIP468 (znuA::lacZ) soxS::Km | 0.96 ± 0.09 |
| SIP775 (znuC::lacZ) soxS::Km | 0.27 ± 0.02 |
| SIP468 (znuA::lacZ) soxR105 | 0.90 ± 0.05 |
| SIP775 (znuC::lacZ) soxR105 | 1.4 ± 0.2 |
| SIP775 (znuC::lacZ)/pSXS-Cma | 5.2 ± 0.5 |
| SIP775 (znuC::lacZ) soxS::Km/pSXS-Cma | 1.8 ± 0.4 |
a
Strains containing pSXS-Cm were grown in the presence of 0.5 mM IPTG and 30 μg/ml chloramphenicol.
b
β-Galactosidase activity values are expressed as a ratio of the LacZ activity of the soxS deletion mutants or overexpressers to that of the wild-type parent, with a value of 1.0 indicating no difference between the two strains. All experiments were performed in triplicate, and the error margins are the standard errors of the mean.