Fig 2.

Growth of E. coli BW25113 after induction of YafQ mutant proteins. Cells with pBADyafQ, pBAD30 and pBADyafQ plasmids with Gln20Ala, Asp61Ala, His87Ala, Phe91Ala, and Phe91Tyr mutations in yafQ gene were grown in liquid LB medium to an A₆₀₀ of 0.1 as described in Materials and Methods. Then synthesis of the proteins was induced by adding 0.2% of l-arabinose (2-h time point). The growth was monitored at the selected time points as the OD₆₀₀ (A) or the CFU/ml (B). The data are means of at least three independent experiments. Bars indicate the standard error.