Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| BL21 | F−ompT (rB− mB−) | 49 |
| BW25113 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-4) lacIp-400(lacIq) λ−rpoS396(Am) rph-1Δ(rhaD-rhaB)568 rrnB-4 hsdR514 | 12 |
| Plasmids | ||
| pBAD30 | Expression plasmid | 17 |
| pBADyafQ | 376-bp fragment with yafQ gene cloned into pBAD30 | 32 |
| pBADyafQQ20A | pBADyafQ with Gln20Ala mutation | This study |
| pBADyafQH50A | pBADyafQ with His50Ala mutation | This study |
| pBADyafQD61A | pBADyafQ with Asp61Ala mutation | This study |
| pBADyafQH63A | pBADyafQ with His63Ala mutation | This study |
| pBADyafQD67A | pBADyafQ with Asp67Ala mutation | This study |
| pBADyafQW68A | pBADyafQ with Trp68Ala mutation | This study |
| pBADyafQW68F | pBADyafQ with Trp68Phe mutation | This study |
| pBADyafQR83A | pBADyafQ with Arg83Ala mutation | This study |
| pBADyafQH87A | pBADyafQ with His87Ala mutation | This study |
| pBADyafQF91A | pBADyafQ with Phe91Ala mutation | This study |
| pBADyafQF91Y | pBADyafQ with Phe91Tyr mutation | This study |
| pBluescript II KS | Expression plasmid | Stratagene |
| pBluescript-hns | 160-bp fragment of the 5′ part of hns gene cloned into pBluescript II (KS) expression vector | This study |
| pAS2 | 1.3-kb DNA fragment with asr gene cloned into pUC19 | 50 |
| pWSK29 | Expression plasmid | 54 |
| pWasr3 | 1.3-kb DNA fragment with asr gene from pAS2, cloned into pWSK29 | This study |
| pRSETdinJyafQ | 559-bp DNA fragment with dinJ and yafQ genes cloned into pRSETb (Invitrogen) | This study |