Fig 1.

Analysis of recombinant Atl expressed from E. coli in an SDS polyacrylamide gel stained with Coomassie brilliant blue R-250. Lane M, protein molecular mass marker; lane 1, crude extract from uninduced cells carrying expression vector; lanes 2, 3, 4, and 5, crude extracts from cells induced for 2, 3, 4, and 5 h, respectively; lane 6, crude extract from cells transformed with an empty expression vector; lane 7, purified 6×His-tagged fusion proteins using a HisTrap FF column. The arrows indicate the recombinant Atl.