Fig 2.

Autolysin profiles of the SDS extracts of the mutant, complementation, and wild-type strains. The recombinant Atl protein was also analyzed by renaturing gel electrophoresis. Lane 1, autolysin profile of the wild-type strain; lane 2, autolysin profile of the mutant strain; lane 3, autolysin profile of atl complementation strain under antibiotic pressure; lane 4, autolysin profile of atl complementation strain without antibiotic pressure; lane 5, purified Atl protein exhibiting lytic activity in a gel containing HA9801 PCW; lane 6, crude extract from E. coli transformed with an empty expression vector; lane M, prestained protein marker. The arrows indicate the autolysins.