Fig 3.

Confirmation of fibronectin-binding capacity of recombinant Atl and Atl expressed in S. suis by Western affinity blotting. Lane 1, SDS extracts of wild-type strain HA9801; lane 2, SDS extracts of the Δatl mutant strain; lane 3, SDS extracts of the Δ atl complement strain; lane 4, crude cell lysate of IPTG-induced E. coli expressing recombinant Atl; lane 5, crude extract from E. coli transformed with an empty expression vector; lane M, prestained protein marker. The arrows indicate signals representing positive fibronectin binding in the Western affinity blot.