Table 1.
Insertional mutants of Pseudomonas syringae with altered surfactant production
| Locus of Tn5 insertion | Predicted function | No. of individual transposon hitsa | Surfactant halo radius (mm)b ± SD | Swarming diam (mm)c ± SD | Swimming diam (mm)d ± SD |
|---|---|---|---|---|---|
| Psyr_3129e (rhlA) | Acyltransferase | 1 | 0 ± 0.0** | 9.0 ± 0.0** | 8.0 ± 1.0 |
| Psyr_3698 (gacS) | Sensor kinase | 1 | 0 ± 0.0** | 9.5 ± 0.6** | 8.3 ± 0.6 |
| Psyr_0258 (ompR) | Response regulator | 1 | 0 ± 0.0** | 9.5 ± 1.0** | 6.0 ± 0.0** |
| Psyr_3461 (fleQ) | NtrC-like flagellar regulator | 1 | 0 ± 0.0** | 9.5 ± 0.7** | 0 ± 0.0** |
| Psyr_4446 (osmE) | Osmotically inducible lipoprotein | 1 | 0.7 ± 0.6** | 14.4 ± 1.5** | 5.3 ± 0.6** |
| Psyr_0936 (wbpY) | O-polysaccharide glycosyltransferase | 3 | 0.7 ± 0.6** | 11.0 ± 0.0** | 0 ± 0.0** |
| Psyr_0219 (algC) | Phosphomannomutase | 1 | 0.7 ± 0.6** | 12.3 ± 2.1** | 1.0 ± 0.0** |
| Psyr_0918 (wzt) | A-band LPS export | 2 | 1.0 ± 0.0** | 11.5 ± 0.6** | 0 ± 0.0** |
| Psyr_2083 | Unknown | 1 | 1.0 ± 0.0** | 16.8 ± 3.4 | 6.3 ± 0.5* |
| Psyr_0270 (polA) | DNA polymerase | 2 | 0.8 ± 0.4** | 13.6 ± 0.8** | 8.0 ± 0.0 |
| Psyr_1981 | PAS:GGDEF | 1 | 1.2 ± 0.4** | 15.3 ± 2.4* | 9.0 ± 1.0 |
| Psyr_3669 | Outer membrane protein | 2 | 1.3 ± 0.6** | 15.8 ± 3.0 | 8.7 ± 0.6 |
| Psyr_3480 (flgC) | Flagellar assembly | 1 | 1.7 ± 0.6** | 10.0 ± 0.0** | 0 ± 0.0** |
| No insertion (ΔsyfA) | 5.3 ± 0.5 | 19.4 ± 2.3 | 8.3 ± 0.6 | ||
| Psyr_3466 (fliC) | Flagellin | 1 | 6.7 ± 0.6** | 10.4 ± 0.5** | 0 ± 0.0** |
| Psyr_3469 (fgt1) | Flagellar glycosyltransferase | 3 | 7.7 ± 1.2** | 9.0 ± 1.0** | 2.0 ± 1.0** |
| Psyr_3468 (fgt2) | Flagellar glycosyltransferase | 1 | 7.0 ± 0.9** | 17.5 ± 2.1 | 8.3 ± 1.5 |
| Psyr_2979 (gor) | Glutathione reductase | 1 | 10.3 ± 1.2** | 27.8 ± 8.9* | 9.0 ± 1.0 |
| Psyr_0263 (algB) | NtrC-like response regulator | 4 | 11.3 ± 1.2** | 26.8 ± 2.3** | 9.7 ± 2.5 |
| Psyr_1350 (mucP) | Peptidase | 1 | 14.3 ± 0.6** | 28.8 ± 6.8** | 9.3 ± 0.6* |
Number of times that independent mutants were identified as insertions in the same gene.
Halos with significantly smaller or larger radii than the halo of the WT (for all, P < 0.01 [**] by a t test).
Bacterial motility across semisolid 0.4% agar plates. The swarming motility was significantly different from the motility of the wild type at P < 0.05 (*) or P < 0.01 (**) as determined by a t test.
Bacterial motility through semisolid 0.25% agar plates. The swimming motility was significantly different from the motility of the wild type at P < 0.05 (*) or P < 0.01 (**) as determined by a t test.
Insertion is immediately upstream of the gene, and the gene name is assigned in this article.