Table 2.

Flagellar assembly classes and their effects on surfactant production

Source	Assemblya	Gene	Relative surfactant production ± SDb
From screen	Class I	fleQ	0 ± 0.00**
Targeted mutant	Class I	fleQ	0 ± 0.00**
Targeted mutant	Class I and IV	fleQ or fliC	0 ± 0.00**
Targeted mutant	Class I	fliA	0.36 ± 0.13**
Targeted mutant	Class II	fliF	0.27 ± 0.16**
From screen	Class III	flgC	0.36 ± 0.13**
Targeted mutant	Class III	flgD	0.33 ± 0.15**
From screen	Class IV	fliC	1.22 ± 0.29*
From screen		fgt1	1.19 ± 0.27*
From screen		fgt2	1.25 ± 0.26**

^aAssembly class designations are given according to those given by Dasgupta et al. (9) for Pseudomonas aeruginosa.

^bRelative surfactant production is expressed as the average surfactant halo radius measured for the mutant divided by the average halo size measured for the ΔsyfA mutant; all averages were calculated from six replicate cultures. Halos were significantly different from the wild type at P < 0.05 (*) or P < 0.01 (**) as determined by a t test.