Table 3.

Relationship between HAA production of transposon mutants and the transcription of rhlA and fliC

Locus of Tn5 insertion	Relative surfactant production ± SDa,d	GFP fluorescence (mean ± SD) of mutants containing the following reporter gene fusion:
		pPrhlA-gfpb,d	pPfliC-gfpc,d
Psyr_3698 (gacS)	0 ± 0.00**	0.60 ± 0.18	+e
Psyr_0258 (ompR)	0 ± 0.00**	0.15 ± 0.08*	+
Psyr_4446 (osmE)	0.13 ± 0.11**	1.09 ± 0.15	+
Psyr_0936 (wbpY)	0.13 ± 0.11**	0.25 ± 0.04*	0.01 ± 0.00**
Psyr_0219 (algC)	0.13 ± 0.11**	1.04 ± 0.23	0.99 ± 0.09
Psyr_0918 (wzt)	0.19 ± 0.02**	0.60 ± 0.18	0.90 ± 0.22
Psyr_2083	0.19 ± 0.02**	0.60 ± 0.14	+
Psyr_0270 (polA)	0.16 ± 0.08**	0.74 ± 0.11	+
Psyr_1981	0.22 ± 0.08**	0.87 ± 0.20	+
Psyr_3669	0.25 ± 0.11**	0.87 ± 0.15	+
Psyr_2979 (gor)	1.93 ± 0.29**	1.09 ± 0.15	+
Psyr_0263 (algB)	2.13 ± 0.30**	1.66 ± 0.40	+
Psyr_1350 (mucP)	2.69 ± 0.28**	2.56 ± 0.66*	+

^aSurfactant production of insertional mutants in a ΔsyfA mutant background is quantified as the radius of surfactant-induced halos in an atomized oil assay divided by the average halo size measured for the ΔsyfA mutant (both values from Table 1).

^bGFP fluorescence of mutants containing the rhlA reporter gene fusion is normalized against that obtained in the ΔsyfA mutant itself.

^cGFP fluorescence of mutants containing the fliC reporter gene fusion is normalized against that obtained in the ΔsyfA mutant itself.

^dValues that were significantly different from those of the wild type as determined by a t test are indicated as follows: *, P < 0.05; **, P < 0.01.

^eExpression levels of flagellin were not investigated for strains with a plus sign, because they exhibited swimming motility.