Table 3.
Growth of trxA, trxC, grxA, and nrdH mutant strainsa
| Strain | Genotype | Complementing plasmid(s) | Growth on medium: |
|
|---|---|---|---|---|
| +ara | +glu | |||
| FA173 | trxA trxC nrdH | pBAD18-trxC | + | + |
| RO36 | trxA trxC grxA nrdH | pBAD18-trxC | + | 0 |
| NK21 | trxA trxC grxA nrdH grxC(M43V) | pBAD18-trxC | + | 0 |
| MAF747 | trxA trxC nrdH | pBAD18-trxC, pBAD33-trxA | + | + |
| MAF750 | trxA trxC grxA nrdH | pBAD18-trxC, pBAD33-trxA | + | 0 |
The indicated strains were grown on medium with arabinose (+ara) to induce expression from the complementing plasmid(s) or with glucose (+glu) to repress expression from the complementing plasmid(s). + indicates that a strain was able to grow, and 0 indicates no growth.