Table 5.
Insertion sequences found in the nrdAB promoter region in E. coli (wild-type and mutant) and Shigella (wild-type) strainsa
| Strain | IS found upstream of nrdAB | Mutations in glutaredoxin and thioredoxin genes |
|---|---|---|
| Shigella flexneri 5 strain 8401 | IS2 | None |
| Shigella boydii Sb227 | IS1 | None |
| Shigella sonnei ss046 | IS2 | None |
| Shigella flexneri 2a strain 301 | IS2 | None |
| Shigella flexneri 2a strain 2457T | IS2 | None |
| Shigella dysenteriae Sd197 | None | None |
| Shigella boydii BS512 | IS1, IS2 | None |
| E. coli MG1655 (wild type) | None | None |
| E. coli MG1655 (RO36) | IS5 | trxA trxC grxA nrdH |
| E. coli MG1655 (NK21) | IS1 | trxA trxC grxA nrdH grxC(M43V) |
a
The nrdAB promoter region was analyzed using MicrobesOnline (4). Glutaredoxin and thioredoxin homologs were identified using BLAST searches with the E. coli proteins against the indicated genomes.