Table 6.
Suppression of the trxA trxC grxA nrdH mutant strain does not require the presence of an IS insertion element in the nrdAB promoter regiona
| Transductant | Growth on medium: |
|
|---|---|---|
| +ara | +glu | |
| MAF750 hda+...Tn10 | + | 0 |
| MAF750 hda(F85V)...Tn10 | + | + |
| MAF750 dnaA+...Tn10 | + | 0 |
| MAF750 dnaA(S203T)...Tn10 | + | + |
| MAF750 dnaA(Q346D)...Tn10 | + | + |
Transductants were tested for their ability to grow on media containing arabinose (+ara), which induces expression of the complementing trxA and trxC, and on media containing glucose (+glu), which represses expression from the complementing plasmids. + indicates that a strain was able to grow (formed large colonies in 1 to 2 days at 37°C) under the indicated condition, while 0 indicates that a strain was unable to grow. The nrdABp allele was checked by PCR, as described in Materials and Methods, and was wild type in each case.