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(A) SDS-PAGE analysis of purified ADP-Glc PPase (I), UDP-Glc PPase (II), and GSase (III) from S coelicolor A3(2) expressed in E. coli BL21(DE3) (UDP-Glc PPase) or E. coli BL21(DE3)/[pGro7] (GSase and ADP-Glc PPase). Left lane corresponds to molecular mass standards. (B) Molecular mass determination, performed from size exclusion chromatography, as detailed in Materials and Methods.
