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. 2012 Mar;194(6):1485–1493. doi: 10.1128/JB.06377-11

Table 1.

Oligonucleotide sequences of the specific primers used to amplify glgC, glgA, and gtaB genes from S. coelicolor A3(2)

Primer Sequencea Restriction site
glgC-fow 5′-TCATATGCTCGGCATCGTGCTGGCGGGCGG-3′ NdeI
glgC-rev 5′-AGAATTCCACGGCACCCGCTGTCCCTTGCCC-3′ EcoRI
glgA-fow 5′-TCATATGCGCGTGGGACTGCTGAGCCGAG-3′ NdeI
glgA-rev 5′-AGAATTCCAAGCCTGCTTGAGGATCTCCTCG-3′ EcoRI
gtaB-fow 5′-TCATATGACTCAGTCCCACCCCAGGATCAGC-3′ NdeI
gtaB-rev 5′-AGAATTCCAACGTCCCTGCATCTCCTCGGC-3′ EcoRI
a

Restriction sites are indicated in bold.