Table 1.
Primer | Sequencea | Restriction site |
---|---|---|
glgC-fow | 5′-TCATATGCTCGGCATCGTGCTGGCGGGCGG-3′ | NdeI |
glgC-rev | 5′-AGAATTCCACGGCACCCGCTGTCCCTTGCCC-3′ | EcoRI |
glgA-fow | 5′-TCATATGCGCGTGGGACTGCTGAGCCGAG-3′ | NdeI |
glgA-rev | 5′-AGAATTCCAAGCCTGCTTGAGGATCTCCTCG-3′ | EcoRI |
gtaB-fow | 5′-TCATATGACTCAGTCCCACCCCAGGATCAGC-3′ | NdeI |
gtaB-rev | 5′-AGAATTCCAACGTCCCTGCATCTCCTCGGC-3′ | EcoRI |
Restriction sites are indicated in bold.