Table 3.
Kinetic parameters for S. coelicolor A3(2) UDP-Glc PPase in a synthetic reactiona
| Substrate | S0.5(mM) | nH | Vmax(U/mg) |
|---|---|---|---|
| TTP | 0.79 ± 0.06 | 1.1 | 94 ± 2 |
| Glc-1P | 0.45 ± 0.02 | 2.4 | 94 ± 2 |
dTTP and Glc-1P were used as substrates. Vmax, S0.5, and nHwere calculated, as detailed in Materials and Methods, from averaged data from three independent experiments, using regression analysis.