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Effect of the multicopy plasmids expressing the RapAC1 or RapCA1 hybrid protein on rapC transcription. Strain JH19278 (spo0A abrB rapC amyE::rapC-lacZ) was transformed with the multicopy plasmids pBS19 (●), pBS19-RapAC1 (▲), and pBS19-RapCA1 (■). Cells were grown in Schaeffer's sporulation medium, and samples were taken at hourly intervals before and after the transition (T0) from the vegetative to the sporulation phase.
