Fig 8.

Time courses of Spo0F∼P dephosphorylation by RapA wt or alanine mutant proteins and inhibition by Phr peptides. Purified Spo0F∼P (0.5 μM) was incubated alone or in the presence of RapA wt or RapA Y224A (A), RapA N225A (B), RapA N228A (C), or RapA H260A (D). The PhrA peptide was added at a 1 μM final concentration. Samples were analyzed by 15% SDS-PAGE and quantitated by the ImageQuant software program after exposure to a PhosphorImager screen. Symbols: no RapA, ●; RapAwt, ▲; RapA wt plus PhrA, ▵-; RapA mutant, ■-; RapA mutant plus PhrA, □-.