Table 1.

Efficiency of sporulation of B. subtilis strains derivative of the parental strain JH642 carrying multicopy plasmids expressing the RapA-RapC hybrid proteins

Straina	Protein expressed	Viable count (cells ml−1)	Spore count (ml−1)	% sporulationb
JH11349	None	2.0 × 108	7.0 × 107	35
JH11169	RapA	1.2 × 108	4.2 × 106	3.5
JH11084	RapC	2.3 × 108	8.9 × 107	38.6
JH19275	RapAC1	1.0 × 107	3.2 × 103	0.032
JH19266	RapCA1	1.1 × 108	4.5 × 107	40.9
JH19276	RapAC2	2.8 × 108	3.9 × 103	0.001
JH19277	RapAC3	2.3 × 108	2.8 × 103	0.001
JH19267	RapAC4	1.0 × 107	3.2 × 103	0.03

^aStrains were grown for 48 h at 37°C in Schaeffer's sporulation medium supplemented with chloramphenicol.

^bData are representative of two independent experiments.