Table 2.

Sporulation efficiency of strains derived from JH642 (parental) expressing the rapA wild type or the chimeric rapA genes in single copy from the chromosome

Straina	Viable count (cells ml−1)	Spore count (ml−1)	% sporulation
JH12834ΔrapA	3.2 × 108	2.25 × 108	70.3
JH642 (parental)	2.1 × 108	1.00 × 108	47.6
JH642::rapAwt	1.8 × 107	6.10 × 105	3.4
JH642::rapAC1	7.5 × 105	1.15 × 104	1.5
JH642::rapAC2	1.59 × 108	1.16 × 108	73.0
JH642::rapAC3	7.8 × 107	5.50 × 107	70.5

^aCells were grown for 48 h at 37°C in Schaeffer's sporulation medium. The data reported are representative of results from two independent experiments.