Table 3.
RT-qPCR analysis of KinB-RpoN regulon genes
| Gene or ORF | Product | Fold change |
|
|---|---|---|---|
| PAO1 kinB ΔrpoN vs PAO1 kinB | PAO1 vs PAO1 kinB | ||
| algU | Sigma factor AlgU | −10.5 | 1.1 |
| mucA | Anti-sigma factor MucA | −8.8 | 1.3 |
| algW | Regulated protease AlgW | −2.9 | −1.5 |
| PA2146 | Conserved hypothetical protein | −11,701.6 | −74.7 |
| katE | Catalase HPII | −6.2 | −2.5 |
| treA | Periplasmic trehalase precursor | −11.7 | 1.4 |
| PA1471 | Extracellular hypothetical | −125.9 | −31.8 |
| pchA | Salicylate biosynthesis isochorismate synthase | −2.2 | −27.0 |
| pchG | Pyochelin biosynthesis protein PchG | 3.2 | 57.7 |
| fptA | Fe(III)-pyochelin outer membrane receptor | 2.2 | 25.5 |
| lecA | LecA PA-I galactophilic lectin | 6.2 | 472.3 |
| aprA | Alkaline metalloproteinase precursor | 12.0 | 34.6 |
| aprD | Alkaline protease secretion protein AprD | 14.4 | 28.7 |
| rhlA | Rhamnosyltransferase chain A | 5.7 | 29.8 |
| rhlB | Rhamnosyltransferase chain B | 27.8 | 114.4 |
| rhlI | Autoinducer synthesis protein RhlI | 7.7 | 21.2 |
| lasB | Elastase LasB | 2.0 | 180.7 |
| lasI | Autoinducer synthesis protein LasI | 1.2 | 1.8 |