Table 5.

Summary of P. aeruginosa gene classes and phenotypes regulated by KinB and RpoN^a

Strain	Alginate activity	Carbohydrateb	Pyochelinc	Relative motilityd	Rhamnolipidse	Virulencef
PAO1	Nonmucoid (wt)	wt	wt	Motile (wt)	wt	Virulent (wt)
kinB strain	Mucoid	↑	↓	Nonmotile	↓	Avirulent
kinB ΔalgU strain	Nonmucoid	↓	ND	Motile	ND	Virulent
kinB ΔrpoN strain	Nonmucoid	↓	↑	Nonmotile	wt	Avirulent

^aUp and down arrows indicated up- and downregulation, respectively; wt, the level of PAO1.

^bHigh numbers of genes which likely play roles in the regulation of carbohydrate metabolism were identified as upregulated in the transcriptome analysis. Expression of these genes was assayed by transcriptome, RT-qPCR, and iTRAQ analyses.

^cExpression of operons and genes which encode products responsible for synthesizing pyochelin was assayed by transcriptome analysis as well as RT-qPCR. ND, not determined.

^dMotility was assayed by various methods (Fig. 3A and B).

^eRhamnolipid levels were measured (Fig. 4A and B). ND, not determined.

^fThe level of virulence of the respective strain to acute infection leading to mortality of BALB/c mice.