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. 2012 Mar;194(6):1342–1349. doi: 10.1128/JB.06411-11

Fig 5.

Fig 5

Enhancement of OnpA activity by hemin and FAD. OnpA activities were determined in a standard enzyme assay (40 nM OnpA, 0.05 mM ONP, 0.2 mM NADPH, and 4 mM Mg2+ in 500 μl [final volume] of phosphate buffer [20 mM, pH 7.5]). The OnpA activity without the addition of FAD or hemin is defined as 100%. The OnpA activity was increased by a further 10% in the presence of both hemin and FAD at a saturation concentration, in comparison with that obtained with hemin alone at a saturation concentration. Values are means ± standard deviations.

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