Fig 5.

DNA release assays. Quantification of eDNA present in supernatants derived from S. mutans UA159, SmuvicK, and Smuvic+ strains using supernatant extracted from early-, mid-log-, and late-growth-phase THYE-grown cultures. Total eDNA was quantified with qRT-PCR using 16S rRNA primers, and the values are expressed as nanograms of eDNA per milligram of dry cell weight. The results indicate mean expression values from three independent experiments, each amplified in triplicate samples ± the standard error.