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Epifluorescence microphotographs of cells from different Ignicoccus species. Bars, 1.5 μm. (A, E, I, and M) Phase-contrast images. (B, F, J, and N) Merged phase-contrast images and SYTO9 fluorescence signals (DNA stain; green). (C, G, K, and O) The localization of the ACS was elucidated by homologous primary antibodies, which were detected by a secondary antibody coupled to Alexa Fluor 546 (red). (D, H, L, and P) Merge of SYTO9 and Alexa Fluor 546 signals.
