Fig 6.

speB mRNA decay in wild type and ΔcvfA strains under different pH conditions. Bacterial strains were grown until early stationary phase, and 1% glucose was added to the culture so that the cells resumed growth. The culture pH was either unbuffered (pH 6.2) (A) or buffered to pH 7.5 (B) or pH 6.0 (C). Rifampin was added immediately after the pH was modified. For Northern blot analysis, 23S rRNA was used as a loading control. One microgram (wild type) or 5 μg (ΔcvfA) of total RNA was loaded per lane.