. 2012 Mar;194(6):1417–1426. doi: 10.1128/JB.06612-11

Table 3.

Estimation of speB mRNA half-lives in different growth phases

Growth phase	Estimated speBmRNA half-life (min)
	qRT-PCR^a	Northern blotting^b
	qRT-PCR^a	Short transcript	Long transcript
Early exponential	3.5 ± 0.3	2.0	0.8
Mid-exponential	7.1 ± 0.6	ND	ND
Late exponential	10.6 ± 1.3	16.3	1.4
Stationary	26.6 ± 2.1	18.1	8.2

Half-life calculated from qRT-PCR analysis in Fig. 2A. The values are means ± standard deviations from two independent experiments.

Half-life calculated from Northern blot analysis in Fig. 2B, C, and D. ND, not determined.