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Active transport characteristics of [3H]tobramycin (2.3 μg/ml) in P. aeruginosa PAO-1. (a) Accumulation occurred in a rapid, time-dependent fashion; (b) uptake at 5 min was concentration dependent; (c) accumulation over 5 min was temperature dependent (cultures grown at 37°C [closed triangle] versus 25°C [closed square]); (d) the electron transport inhibitor NaCN (1 mM) blocked uptake, demonstrating energy dependence; (e) uptake was inhibited by the divalent cation MgCl2 (1 mM).
