Fig 2.

Antileishmanial potency of 123859 against intracellular L. donovani as determined by Giemsa staining of LV82-infected murine peritoneal macrophages. (A) Infected macrophage control; (B) noninfected macrophage control; (C) infected macrophages treated with 3 nM 123859; (D) infected macrophages treated with 24 nM 123859 (which inhibits about 50% of parasitemia as confirmed by microscopic enumeration); (E) infected macrophages treated with 500 nM 123859 (which clears parasitemia); (F) antileishmanial dose-response curve for 123859 from a representative experiment as assessed by microscopic enumeration. Infection ratio was calculated as described by Siqueira-Neto et al. (28). Bar, 10 μm.