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Kinetics of cefamandole hydrolysis. Absorption at 285 nm of H37Rv (●) or H37RvΔrpfACDEB (■). Samples were mechanically lysed (A) or resuspended in phosphate-buffered saline intact (B) and exposed to 20 mM cefamandole nafate. Subsequently, absorption measurements were taken at the indicated times. The illustrated values are averages of two technical replicates for each strain. Parameters vlysed and vintact are the slope and error calculated from the aggregated data for each strain.
