Table 2.
IC90s of β-lactams against H37Rv, RPFnull, and related strains
| β-Lactam | Straina | IC90 (μM)b |
|---|---|---|
| Cefotaxime | H37Rv (RPFWT) | 26 |
| H37RvΔrpfA | 13–26 | |
| H37RvΔrpfB | 13–26 | |
| H37RvΔrpfC | 26 | |
| H37RvΔrpfD | 26 | |
| H37RvΔrpfE | 26–52 | |
| H37RvΔrpfAB | 26 | |
| H37RvΔrpfACBED::pHrpfCDE | 6.5–26 | |
| H37RvΔrpfACBE | 6.5 | |
| H37RvΔrpfACBED (RPFnull) | 1.6–3.3 | |
| H37RvΔrpfACDEBc | 0.78 | |
| Cefamandole | H37Rv (RPFWT) | 390–780 |
| H37RvΔrpfACBED (RPFnull) | 98 | |
| With potassium clavulanate (1 μM) | H37Rv (RPFWT) | 24 |
| H37RvΔrpfACBED (RPFnull) | 1.5 | |
| Ceftriaxone | H37Rv (RPFWT) | 3.2–6.3 |
| H37RvΔrpfACBED (RPFnull) | 1.6 |
a
From reference 5.
b
Differences in IC90s are representative of at least two independent experiments containing at least four duplicates each; for cefamandole, amounts of clavulanate were varied across duplicates.
c
RPFs were deleted from this strain in a different order than that for RPFnull.