Skip to main content
. 2012 Mar;56(3):1630–1634. doi: 10.1128/AAC.05729-11

Table 1.

Pre-steady-state rate constants for dNTP incorporation by Pol γa

Nucleotide kpol (s−1) Kd (μM) Efficiency (μM−1 s−1)b Discriminationc
dATP 220 ± 16 3.2 ± 0.7 69
EFdA-TP 0.29 ± 0.02 18 ± 4 0.016 4,300
a

At least 7 different time courses containing 10 time points each were used to generate kpol and Kd. The standard error estimates shown were derived from the deviance from the nonlinear regression fits determined using KaleidaGraph software.

b

Efficiency = kpol/Kd.

c

Discrimination = efficiency(dATP)/efficiency(EFdA-TP).