Fig 2.

Growth curves of E. coli ATCC 35318 (A), P. aeruginosa ATCC 27853 (B), E. coli 5539 (C), and P. aeruginosa 5215 (D) cells treated with different concentrations of battacin. The curves are viable cell concentrations plotted against time. Open squares, nonbattacin control; closed squares, 0.5× MIC of battacin; closed triangles, 1× MIC of battacin; closed diamonds, 4× MIC of battacin; open diamonds, polymyxin B (16 mg/liter). For the four strains in the present study, time-kill assays were independently performed 2 to 4 times, and similar results were obtained. Mean values of the triplicate CFU/ml measurements from a single experiment are plotted.