Table 2.
Susceptibility of 1b replicon variants to BMS-790052a
| Replicon variant | Replication level | Transient assay |
Cell line |
||
|---|---|---|---|---|---|
| EC50 (nM) | FR | EC50 (nM) | FR | ||
| WT | 100 | 0.0026 ± 0.0009 | 1.0 | 0.0031 ± 0.0016 | 1.0 |
| L31V | 157.9 ± 53.6 | 0.072 ± 0.021 | 28 | 0.25 ± 0.016 | 81 |
| L31W | 190.7 ± 9.1 | 0.210 ± 0.002 | 81 | ND | ND |
| P32L | 17.5 ± 5.6 | 0.042 ± 0.021 | 16 | ND | ND |
| P58S | 120.5 ± 17.9 | 0.0023 ± 0.0004 | 0.9 | ND | ND |
| Y93C | 61.9 ± 4.4 | 0.009 ± 0.002 | 3.5 | ND | ND |
| Y93H | 26.9 ± 15.5 | 0.062 ± 0.025 | 24 | 0.45 ± 0.047 | 145 |
| Y93N | 18.8 ± 5.2 | 0.13 ± 0.088 | 50 | ND | ND |
| L31V+Y93H | 49.9 ± 38.0 | 38.0 ± 33.4 | 14615 | 250 ± 100 | 80645 |
a
Replication level, mean ± standard deviation relative to the wild type as determined from transient assays with the indicated replicons (n ≥ 3). EC50, mean ± standard deviation (n ≥ 3); WT, wild type; FR, fold resistance; ND, not determined.