Table 3.
Amino acid substitutions predicted from bulk NS5A cDNA recovered from genotype 1a replicon cells surviving BMS-790052 treatmenta
| Amino acid substitution | % amino acid substitutions predicted for indicated concn of BMS-790052 (nM) and treatment duration (days) |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
10 |
100 |
250b |
500c |
|||||||||
| 3 | 7 | 14 | 3 | 7 | 14 | 3 | 7 | 14 | 3 | 7 | 3 | 7 | |
| M28T | 20 | 25 | 20 | 25 | 30 | 30 | 5 | 40 | |||||
| Q30D | 5 | ||||||||||||
| Q30E | 5 | 40 | 50 | 50 | 65 | 100 | 70 | 50 | |||||
| Q30H | 25 | 35 | 40 | 40 | 35 | 50 | 15 | ||||||
| Q30K | 20 | 10 | 30 | ||||||||||
| Q30R | 10 | 15 | 20 | 10 | 5 | 5 | |||||||
| L31M | 5 | 5 | 5 | 5 | 5 | ||||||||
| H58D | 5 | ||||||||||||
| Y93H | 5 | 10 | 10 | 10 | 20 | 25 | 30 | 25 | 40 | ||||
| Y93N | 5 | 5 | 10 | ||||||||||
a
Values in the table are estimated percentages based on visual inspection of the population cDNA sequence chromatogram.
b
A few colonies were observed following 14 days of treatment, but these were not successfully amplified.
c
No replicon cells survived 14 days of treatment.