Table 2.

bla_KPC excision patterns in additional Tn4401-bearing strains

Strain	Species	InC type(s)a	MLSTb	KPC	Tn4401 variantc	Size(s) of Tn4401 deletion pattern(s) (bp)d
1	K. pneumoniae	N, FIIs	63	KPC-4	b	160, 278, 430
2	K. pneumoniae	Unk	76	KPC-3	a	155, 217
3	K. pneumoniae	FIIs	234	KPC-2	a	289
4	K. pneumoniae	FIIs	258	KPC-2	a	278, 430
5	K. pneumoniae	FIIs	258	KPC-3	b	186, 278, 430
6	K. pneumoniae	N, A/C	258	KPC-2	a	215, 278, 430
7	K. pneumoniae	Unk	258	KPC-2	a	191, 278, 430
8	K. pneumoniae	FIIs	258	KPC-3	b	278, 430
9	K. pneumoniae	FIIs	258	KPC-3	−68	255
10	K. pneumoniae	A/C	486	KPC-2	b	278, 430
11	E. coli	L1, N, Y, FIA, FIB, FIIs	2	KPC-2	b	278, 430
12	E. coli	L1, N, Y, FIA, FIB, FIIs	2	KPC-3	b	278, 430
13	E. coli	L1, FIA, FIB, FIIs	43	KPC-2	a	278, 430
14	E. coli	FIB, A/C, Frep	33	KPC-2	a	278, 430
15	E. coli	A/C	35	KPC-3	b	278

^aPlasmid replicon typing was performed using a method previously described by Carattoli et al. (2). Unk, unknown.

^bMLST for K. pneumoniae was performed using the method described by Diancourt et al. (9); MLST for E. coli was performed using the Institut Pasteur MLST scheme (http://www.pasteur.fr/recherche/genopole/PF8/mlst/EColi.html).

^cTn4401 variants were identified by PCR (6), followed by DNA sequencing. “−68” indicates the previously described variant with a 68-bp deletion upstream of bla_KPC (13).

^dTn4401 deletion patterns were identified by nested PCR and are expressed as the PCR product length resulting from amplification with primer set F2/R2.