Fig 3.

Immunohistochemical analysis of the cornea and ciliary body of rats. Panels a to f show results 1 h after 5 instillations a day of 10 μg/ml AC-8 for 4 days and then a single instillation on day 4 (group 3; a to c, central cornea, and d to f, peripheral cornea). Insets show higher magnifications. Panels j to l show results 1 h after 5 instillations a day of PBS over 4 days and then a single instillation on day 4 (vehicle; group 6, j to l). Panels g to i show ciliary body with the same AC-8 treatment (group 3). Panels m to o show control PBS-treated ciliary body (group 6). Left panels show DAPI staining (blue), middle panels show Alexa-labeled anti human IgG antibody (red), and right panels show the merged images. Positively stained dendritiform subepithelial cells are indicated by arrowheads. Bar, 50 μm.