Fig 4.

Immunohistochemical analysis of the rat retina. Panels a to d show results 1 h after 5 instillations a day of 10 μg/ml AC-8 for 4 days and then a single instillation on day 4 (group 3). Panels e to g show results 1 h after 5 instillations a day of PBS for 4 days and then a single instillation on day 4 (vehicle; group 6). Panel d is a higher magnification of the dashed white square in panel c. The left panels show DAPI staining (blue), the middle panels show Alexa-labeled anti-human IgG antibody (red), and the right panels show the merged images. Arrowheads point to positively stained astrocytes. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Bar, 100 μm.