Fig 5.

Immunohistochemical analysis of the rat eye 1 h after one subconjunctival injection. Shown is cornea 1 h after one subconjunctival injection of 10 μg/ml AC-8 (group 4; a to c) or PBS (group 7; d to f). White arrows indicate positive anti-human IgG staining. Panels g to t show results for ciliary body (g to l) and retina (m to t) 1 h after subconjunctival injection of AC-8 (group 4; g to i, m to o, and s and t) or PBS (group 7, j to l and p to r). Panels s and t are two higher magnifications of the white dashed squares in panel o. In panel s, arrowheads indicate stained astrocytes in the inner part of the retina, and white arrows indicate stained Müller cell prolongations. The left column shows DAPI staining, the middle column shows Alexa-labeled anti-human IgG antibody, and the right column shows the merged images.