Fig 5.

IRF1 and IRF7 recruitment to the Gbp2 promoter after treatment with IFN-γ. (A) Schematic drawing of the GAS located in the distal region and ISRE sites located in both the distal and proximal promoter regions of the Gbp2 gene. (B to J) WT MEFs, Tbk1/Ikbke^−/− MEFs (H and I), or Irf7^−/− MEFs were treated with IFN-γ for the time periods indicated. The cells were processed for ChIP (B and E) or ChIP-reChIP (C, D, and F to J) with the antibodies shown on top of the panels. The precipitates were amplified with primers flanking the distal (B to D) or proximal (E to J) Gbp2 promoter and were analyzed by qPCR. Data are expressed as percentages of precipitate relative to input DNA. qPCR measurements were made in triplicate. All experiments were repeated at least three times.