Fig 3.

RYBP represses endogenous retroviruses. (A) Upregulation of MuERV mRNAs among those encoded by LTR-retroelements measured by RT-qPCR of ES cells after 4 days of 4′-OHT treatment. (B and C) Schematic representation of MuERV retroelements and expression. RT-qPCR expression analysis of indicated regions. LTR, long terminal repeat; Gag, Pro-pol, and dUTPase, gene region encoding a Gag-Pro dUTPase polyprotein; PBS, primer binding site; PPT, polypurine tract. Means and SDs of three experiments are shown. (D) Lsd1 levels in wild-type and RYBP-mutant ES cells.