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. 2012 Mar;32(6):1112–1123. doi: 10.1128/MCB.06511-11

Fig 5.

Fig 5

The interaction of Integrator 9 and Integrator 11 is required for snRNA 3′ end formation. (A) Western blot analysis of lysates from cells treated with either control siRNA or siRNA targeting IntS11 and then transfected with HA-tagged IntS11 or HA-tagged IntS11 that is resistant to the IntS11 siRNA. (B) Western blot analysis using anti-IntS11 antibodies, demonstrating expression of exogenous RNAi-resistant IntS11 comparable to that of the endogenous IntS11. (C) Schematic of human U7-GFP reporter construct that produces GFP only in response to misprocessing conditions when Integrator subunits are knocked down. Right panel, Western blot analysis of cell lysates from cells treated with either control siRNA or IntS11 siRNA that were transfected with empty vector (VA) or RNAi-resistant IntS11 (lanes 11*) along with U7-GFP reporter. (D) Western blot analysis of cell lysates from cells treated with control siRNA or with IntS11 siRNA followed by transfection with the U7-GFP reporter and either full-length RNAi-resistant IntS11 or RNAi-resistant fragments as described for Fig. 2A.