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Effect of TPK2 overexpression on growth of the BCY1 null strain. A C. albicans TPK1/TPK1 tpk2/tpk2 bcy1/bcy1 mutant was transformed with plasmid pLS4. Four different transformants were tested (•, ⧫, ▴, ▾). The control strain was CAI4 transformed with plasmid pBI (▪). Strains were grown in noninducing medium SD (A) and in CSAA inducing medium (B) at 30°C with agitation (150 rpm). The optical density of the cultures was measured at 600 nm.
