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Growth of strains CAI4, H2D, and bcy1 tpk2. Cells were grown in liquid YPD supplemented with uridine (50 μg/ml). The optical density at 600 nm (OD600) of the cultures was adjusted to 0.1 with the same medium, and 5-μl aliquots from the cultures and from 10-fold serial dilutions were spotted onto YPD plates supplemented with uridine (50 μg/ml). Plates were incubated at 30°C for 2 days.
