TABLE 1.
Strains and plasmids used in this study
Strain or plasmid | Characteristics | Source or reference |
---|---|---|
C. albicans strains | ||
CAI4 | ura3::λimm434/ura3::λimm434 | 11 |
H2D | ura3::λimm434/ura3::λimm434 Δtpk2::cat/Δtpk2::cat | 7 |
bcy1 tpk2 | ura3::λimm434/ura3::λimm434 Δtpk2::cat/Δtpk2::cat bcy1/bcy1 | This study |
ASM1 | ura3::λimm434/ura3::λimm434 TPK1/TPK1-GFP-URA3 | This study |
ASM2 | ura3::λimm434/ura3::λimm434 Δtpk2::cat/Δtpk2::cat TPK1/TPK1-GFP-URA3 | This study |
ASM3 | ura3::λimm434/ura3::λimm434 Δtpk2::cat/Δtpk2::cat bcy1/bcy1 TPK1/TPK1-GFP-URA3 | This study |
Plasmids | ||
pBI-1 | PCK1 promoter in URA3-marked CARS2 vector | J. F. Emst |
pLS4 | PCK1 promoter controlling a His-tagged TPK2 in pRC2312 | E. Setiadi J. F. Ernst |