TABLE 2.
Results of transformation experiments performed to delete both alleles of BCY1 in C. albicans strain CAI4
| Expected genotype (plasmid)a | No. of transformants tested by Southern analysis | No. of transformants of the expected genotype |
|---|---|---|
| BCY1/Δbcy1::cat-URA3-cat (pMP1) | 9 | 2 |
| Δbcy1::cat-URA3-cat/Δbcy1::cat (pMP1) | 113 | 0 |
| bcy1::cat-URA3-cat/Δbcy1::cat (pMP3) | 20 | 0 |
| BCY1/Δbcy1::cat-URA3-cat Δtpk2::cat/Δtpk2::cat (pMP1) | 16 | 5 |
| Δbcy1::cat-URA3-cat/Δbcy1::cat Δtpk2::cat/Δtpk2::cat (pMP1) | 31 | 9 |
a
The transformations were performed with the indicated plasmid.