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. 2004 Feb;3(1):108–120. doi: 10.1128/EC.3.1.108-120.2004

FIG. 1.

FIG. 1.

mob2Δ confers a synthetic slow-growth phenotype to a ras2Δ strain. (A) Strain Y3011 (ras2 mob2 [CEN-RAS2-URA3]) harboring, from top to bottom, [CEN-LEU2], [CEN-LEU2-RAS2], [CEN-HIS3], or [CEN-HIS3-MOB2] were grown in SC liquid medium, spotted onto SC and SC plus FOA plates in fivefold serial dilutions, and incubated at 30°C. (B) Tetrad analysis of Y3012 (mob2Δ) × Y3013 (ras2Δ). A representative tetratype tetrad is labeled and shown at the left of the tetrads. At least 10 tetrads were analyzed with consistent results (mob2Δ ras2Δ spore clones are circled). (C) cbk1Δ, hym1Δ, pag1Δ, and kic1Δ confer a synthetic slow-growth phenotype with ras2Δ. In all subpanels, representative tetratype tetrads are labeled and shown. For each strain, at least 10 tetrads were analyzed with results fully consistent with those shown. (Left to right) Tetrad analysis of Y3013 (ras2Δ) × Y3016 (cbk1Δ), Y3013 (ras2Δ) × Y3027 (hym1Δ), Y3090 (ras2Δ) × Y3088 (pag1Δ), and Y2813 (ras2Δ) × Y3089 (kic1Δ).